comparison of embryo development between intracytoplasmic and piezo-assisted sperm injection after treating mouse sperms by ca2+ ionophore

نویسندگان

عبدالحسین شاهوردی

abdolhossein shahverdi منصوره موحدین

mansoureh movahedin مجتبی رضازاده ولوجردی

mojtaba rezazadeh valojerdi سعید کاظمی آشتیانی

saeid kazemi ashtiani

چکیده

the purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (icsi) and piezo-assisted sperm injection after pretreatment with calcium ionophore (cai) on the mouse embryo development. methods: in this study, the conventional icsi and piezo-icsi procedures were used. the efficacy of the methods was examined after mouse matured oocytes were fertilized with or without cai-treated sperms. results: piezo-icsi demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional icsi: 42%, p<0.001) and a cleavage rate of 73% (conventional icsi: 58%, p<0.05). when the piezo-icsi procedure was performed with cai-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, p<0.05). however, the fertilization rate did not change significantly (64% vs. 56%). conclusion: the piezo-icsi accompanies with cai-treated sperms is more efficient than the conventional icsi method for fertilizing and thus obtaining more mouse embryos.

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Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca2+ Ionophore

The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

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Comparison of embryo development between intracytoplasmic and Piezo-assisted sperm injection after treating mouse sperms by Ca2+ ionophore.

BACKGROUND The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. METHODS In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized w...

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Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca Ionophore

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Piezo-actuated Mouse ICSI (Intracytoplasmic sperm injection)

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عنوان ژورنال:
iranian biomedical journal

جلد ۱۱، شماره ۴، صفحات ۲۴۵-۲۵۰

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